Thursday, May 17, 2007
12:53 PM
PCR
cDNA from: 05/14/07
1?L of each primer
|
cDNA |
Labeled |
PRIMERS |
|
1A – 4.00?L |
|
Bactin Sense & Antisense |
|
1B – 3.03?L |
|
” “ |
|
2A – 2.45?L |
|
” “ |
|
2B – 1.96?L |
|
” “ |
|
3A – 1.73?L |
|
” “ |
|
3B – 1.97?L |
|
” “ |
|
6A – 1.46?L |
|
” “ |
|
6B – 1.60?L |
|
” “ |
|
7A – 1.19?L |
|
” “ |
|
7B – 1.38?L |
|
” “ |
|
8A – 1.18?L |
|
” “ |
|
8B – 1.29?L |
|
” “ |
|
9A – 2.51?L |
|
” “ |
|
9B – 2.58?L |
|
” “ |
Up to 25?L of Nuclease Free Water
12.5?L GoTaq Green Master Mix 2x (Promega)



















































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