TOC
Once I get all the research pages published to the site, I will create a flow chart.
Rather than chronological order (as the pages are in the sidebar menu), this will outline the research flow and results with links to the actual pages.
Example
Experiment 10
FSF Cells
Treated(R2-27)
10A – Control
10B – Infected w/ y4
10C – Infected w/ y5
10D – Infected w/ y7
Harvested(R2-31)
RNA/Protein Extraction(R2-32)
BCA Assay(R2-34)
RNA Quantitation/RT(R2-36)
PCR
Beta Actin – 60°/17cycles(R2-37)
Agarose Gel – 2% – Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-38)
Agarose Gel – 2% – Gel A Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-41)
Total PPARy(Primers G & H) – 65°/22cycles(R2-40)
Agarose Gel – 2% – Gel B Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-41)
Agarose Gel – 2% – Gel A Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-47)
Total PPARy(Primers G & H) – 65°/22cycles(R2-46)
Agarose Gel – 2% – Gel B Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-47)
V5 tag from virus (Primers V5 & S2V5) – 68°/34cycles(R2-43)
Agarose Gel – 2% – Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-45)
PPARy Isoforms (Primers F & I) – 58°/35cycles(R2-48)
Agarose Gel – 2% – Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-49)
Agarose Gel – 2% – Gel C Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-51)
PPARy Isoforms (Primers A & I) – 52°/35cycles(R2-50)
Agarose Gel – 2% – Gel A&B Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-51)
PPARy Isoforms (Primers A & I) – 54°/30cycles(R2-53)
Agarose Gel – 2% – Gel A&B Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-55)
PPARy Isoforms (Primers A & I) – 56°/26cycles(R2-57)
Agarose Gel – 2% – Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-58)
PPARy Isoforms (Primers A & I, F & I) – 60°/29cycles(R2-59)
Agarose Gel – 2% – (Gel A-A&I, Gel B-F&I) Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-60)
Protein Gel
12% SDS-PAGE – Membrane transfer(R2-39)
Western Blot – PPAR – Proteins didn’t enter gel?(R2-42)
Western Blot – Beta Actin – Proteins didn’t enter gel?(R2-44)
10% SDS-PAGE – Membrane transfer(R2-52)
Western Blot – PPAR – Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-54)
Western Blot – Beta Actin – Lanes(1=Control, 2=y4, 3=y5, 4=y7)(R2-56)
Experiment 11
THP-1 Cells
Treated(R2-12)
11A – Control
11B – Infected w/ y4
11C – Infected w/ y5
11D – Infected w/ y7
Harvested(R2-15)
RNA/Protein Extraction(R2-16)
BCA Assay(R2-28)
RNA Quantitation/RT(R2-36)
PCR
Beta Actin – 60°/17cycles(R2-37)
Agarose Gel – 2% – Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-38)
Agarose Gel – 2% – Gel A Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-41)
Total PPARy(Primers G & H) – 65°/22cycles(R2-40)
Agarose Gel – 2% – Gel B Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-41)
Agarose Gel – 2% – Gel A Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-47)
Total PPARy(Primers G & H) – 65°/22cycles(R2-46)
Agarose Gel – 2% – Gel B Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-47)
V5 tag from virus (Primers V5 & S2V5) – 68°/34cycles(R2-43)
Agarose Gel – 2% – Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-45)
PPARy Isoforms (Primers F & I) – 58°/35cycles(R2-48)
Agarose Gel – 2% – Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-49)
Agarose Gel – 2% – Gel C Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-51)
PPARy Isoforms (Primers A & I) – 52°/35cycles(R2-50)
Agarose Gel – 2% – Gel A&B Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-51)
PPARy Isoforms (Primers A & I) – 54°/30cycles(R2-53)
Agarose Gel – 2% – Gel A&B Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-55)
PPARy Isoforms (Primers A & I) – 56°/26cycles(R2-57)
Agarose Gel – 2% – Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-58)
PPARy Isoforms (Primers A & I, F & I) – 60°/29cycles(R2-59)
Agarose Gel – 2% – (Gel A-A&I, Gel B-F&I) Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-60)
Protein Gel
12% SDS-PAGE – Membrane transfer(R2-39)
Western Blot – PPAR – Proteins didn’t enter gel?(R2-42)
Western Blot – Beta Actin – Proteins didn’t enter gel?(R2-44)
10% SDS-PAGE – Membrane transfer(R2-52)
Western Blot – PPAR – Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-54)
Western Blot – Beta Actin – Lanes(5=Control, 6=y4, 7=y5, 8=y7)(R2-56)
