Onenote Web Exporter – Full Interactive PPAR lab notebook
Check out the new (to me) Onenote web exporter.
I have my full lab notebook here. LINK
This should give a little better feel to people about how Onenote can be used.
You are currently browsing the ELN - PPAR gamma category.
R2-81
Monday, May 21, 2007
12:46 PM
PCR
cDNA from: 05/14/07
1μL of each primer
|
cDNA |
Labeled |
PRIMERS |
|
1A – 4.00μL |
|
A, I, F |
|
1B – 3.03μL |
|
” “ |
|
2A – 2.45μL |
|
” “ |
|
2B – 1.96μL |
|
” “ |
|
3A – 1.73μL |
|
” “ |
|
3B – 1.97μL |
|
” “ |
|
6A – 1.46μL |
|
” “ |
|
6B – 1.60μL |
|
” “ |
|
7A – 1.19μL |
|
” “ |
|
7B – 1.38μL |
|
” “ |
|
8A – 1.18μL |
|
” “ |
|
8B – 1.29μL |
|
” “ |
|
9A – 2.51μL |
|
” “ |
|
9B – 2.58μL |
|
” “ |
Up to 25μL of Nuclease Free Water
12.5μL GoTaq Green Master Mix 2x (Promega)
Run PCR File 76
Adjusted Annealing/Cycles:61C, 31cyc
Posted 3 years, 3 months ago at 12:46 pm. Add a comment
R2-79
Thursday, May 17, 2007
12:53 PM
PCR
cDNA from: 05/14/07
1μL of each primer
|
cDNA |
Labeled |
PRIMERS |
|
1A – 4.00μL |
|
Bactin Sense & Antisense |
|
1B – 3.03μL |
|
” “ |
|
2A – 2.45μL |
|
” “ |
|
2B – 1.96μL |
|
” “ |
|
3A – 1.73μL |
|
” “ |
|
3B – 1.97μL |
|
” “ |
|
6A – 1.46μL |
|
” “ |
|
6B – 1.60μL |
|
” “ |
|
7A – 1.19μL |
|
” “ |
|
7B – 1.38μL |
|
” “ |
|
8A – 1.18μL |
|
” “ |
|
8B – 1.29μL |
|
” “ |
|
9A – 2.51μL |
|
” “ |
|
9B – 2.58μL |
|
” “ |
Up to 25μL of Nuclease Free Water
12.5μL GoTaq Green Master Mix 2x (Promega)
Posted 3 years, 3 months ago at 12:53 pm. Add a comment
R2-77
Tuesday, May 15, 2007
2:55 PM
PCR
cDNA from: 05/14/07
|
cDNA |
Labeled |
PRIMERS |
|
1A |
|
Bactin Sense & Antisense |
|
1B |
|
” “ |
|
2A |
|
” “ |
|
2B |
|
” “ |
|
3A |
|
” “ |
|
3B |
|
” “ |
|
6A |
|
” “ |
|
6B |
|
” “ |
|
7A |
|
” “ |
|
7B |
|
” “ |
|
8A |
|
” “ |
|
8B |
|
” “ |
|
9A |
|
” “ |
|
9B |
|
” “ |
Up to 25μL of Nuclease Free Water
12.5μL GoTaq Green Master Mix 2x (Promega)
Posted 3 years, 3 months ago at 2:55 pm. Add a comment
R2-75
Monday, May 07, 2007
5:53 PM
Protein Gel
|
Lane |
|
μL |
μL H2O |
|
1 |
LMW |
7 |
|
|
2 |
γ1 |
5 |
3+2 5x buffer |
|
3 |
γ2 |
5 |
3+2 5x buffer |
|
4 |
γ4 |
5 |
3+2 5x buffer |
|
5 |
γ5 |
5 |
3+2 5x buffer |
|
6 |
γ7 |
5 |
3+2 5x buffer |
|
7 |
XEF |
5 |
3+2 5x buffer |
|
8 |
B |
5 |
3+2 5x buffer |
|
9 |
blank |
10 |
|
|
10 |
HMW |
7 |
|
|
y1 |
112.0533 |
|
y2 |
100 |
|
y4 |
31.37921 |
|
y5 |
20.68615 |
|
y7 |
25.66406 |
|
|
|
|
y1+ |
92.30844 |
|
y2+ |
100 |
|
y4+ |
96.20047 |
|
y5+ |
83.76292 |
|
y7+ |
86.24552 |
|
XEF |
91.36397 |
Posted 3 years, 4 months ago at 5:53 pm. Add a comment
R2-74
Saturday, May 05, 2007
4:14 PM
In Vitro Transcription-Translation
PR15
DNA template from: γ1 5/4 (4μL) – γ7 5/4, pTRI-Xef(1μL), Blank
Transcription Products: T γ1 – T γ7, T γ1+ – T γ7+ (DNA template & XEF template), T XEF, T B
Translation Products:x2
IVT γ1 – IVT γ7,IVT γ1+ – IVT γ7+, IVT XEF, IVT B
